Contact an Account ManagerComparison of the separation of steroids on NUCLEOSHELL RP 18 and on NUCLEODUR® C18 Gravity
Summary
Type: HPLC
Application #: 124930
Catalog#: 763132.30 - MN HPLC Column, Nucleoshell RP 18, 90A, 2.7um, 3x50mm
Detection:
Conditions:
NUCLEOSHELL RP 18 NUCLEODUR C18 Gravity
Eluent A: acetonitrile acetonitrile
Eluent B: water water
Gradient: 30–80 % A in 1.0 min,
hold for 0.5 min 30–80 % A in 5.0 min,
hold for 2.0 min
Flow: 2.0 mL/min 1.0 mL/min
Temperature: 25 °C 25 °C
Pressure: 350 bar 280 bar
Injection volume: 1.0 µL 1.0 µL
Authors: HPLC department
Source: Macherey-Nagel, Germany 2011
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